Anesthesiology and Pain Medicine

Published by: Kowsar

The Relationship Between Creatine and Whey Protein Supplements Consumption and Anesthesia in Rats

Kianoush Saberi 1 , * , Mohammad Amin Gorji Mahlabani 2 , Mohammad Tashayoie 2 and Farinaz Nasiri Nejad 3
Authors Information
1 Anesthesiology Department, Imam Khomeini Medical and Research Center, Tehran University of Medical Sciences, Tehran, Iran
2 Under Graduate Department, Student’s Scientific Research Center (SSRC), Exceptional Talent Development Center (ETDC), Tehran University of Medical Sciences, Tehran, Iran
3 Physiology Department, Iran University of Medical Sciences, Tehran, Iran
Article information
  • Anesthesiology and Pain Medicine: February 01, 2016, 6 (1); e32648
  • Published Online: February 13, 2016
  • Article Type: Research Article
  • Received: August 24, 2015
  • Revised: October 28, 2015
  • Accepted: November 14, 2015
  • DOI: 10.5812/aapm.32648

To Cite: Saberi K, Gorji Mahlabani M A, Tashayoie M, Nasiri Nejad F. The Relationship Between Creatine and Whey Protein Supplements Consumption and Anesthesia in Rats, Anesth Pain Med. 2016 ; 6(1):e32648. doi: 10.5812/aapm.32648.

Copyright © 2016, Iranian Society of Regional Anesthesia and Pain Medicine (ISRAPM). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Chatterton DE, Nguyen DN, Bering SB, Sangild PT. Anti-inflammatory mechanisms of bioactive milk proteins in the intestine of newborns. Int J Biochem Cell Biol. 2013; 45(8): 1730-47[DOI][PubMed]
  • 2. Ebaid H, Ahmed OM, Mahmoud AM, Ahmed RR. Limiting prolonged inflammation during proliferation and remodeling phases of wound healing in streptozotocin-induced diabetic rats supplemented with camel undenatured whey protein. BMC Immunol. 2013; 14: 31[DOI][PubMed]
  • 3. Kui B, Balla Z, Vegh ET, Pallagi P, Venglovecz V, Ivanyi B, et al. Recent advances in the investigation of pancreatic inflammation induced by large doses of basic amino acids in rodents. Lab Invest. 2014; 94(2): 138-49[DOI][PubMed]
  • 4. Hensley CT, Wasti AT, DeBerardinis RJ. Glutamine and cancer: cell biology, physiology, and clinical opportunities. J Clin Invest. 2013; 123(9): 3678-84[DOI][PubMed]
  • 5. Wischmeyer PE, Dhaliwal R, McCall M, Ziegler TR, Heyland DK. Parenteral glutamine supplementation in critical illness: a systematic review. Crit Care. 2014; 18(2)[DOI][PubMed]
  • 6. Scrimgeour AG, Condlin ML. Nutritional treatment for traumatic brain injury. J Neurotrauma. 2014; 31(11): 989-99[DOI][PubMed]
  • 7. Brandelli A, Daroit DJ, Corrêa APF. Whey as a source of peptides with remarkable biological activities. Food Res Int. 2015; 73: 149-61[DOI]
  • 8. Ha E, Zemel MB. Functional properties of whey, whey components, and essential amino acids: mechanisms underlying health benefits for active people (review). J Nutr Biochem. 2003; 14(5): 251-8[PubMed]
  • 9. Millward DJ. Knowledge gained from studies of leucine consumption in animals and humans. J Nutr. 2012; 142(12): 2212S-9S[DOI][PubMed]
  • 10. Cribb PJ, Williams AD, Stathis CG, Carey MF, Hayes A. Effects of whey isolate, creatine, and resistance training on muscle hypertrophy. Med Sci Sports Exerc. 2007; 39(2): 298-307[DOI][PubMed]
  • 11. Tavares TG, Spindola H, Longato G, Pintado ME, Carvalho JE, Malcata FX. Antinociceptive and anti-inflammatory effects of novel dietary protein hydrolysate produced from whey by proteases of Cynara cardunculus. Int Dairy J. 2013; 32(2): 156-62[DOI]
  • 12. Bhargava HN, Sharma SS, Bian JT. Evidence for a role of N-methyl-D-aspartate receptors in L-arginine-induced attenuation of morphine antinociception. Brain Res. 1998; 782(1-2): 314-7[PubMed]
  • 13. Qi X, Evans AM, Wang J, Miners JO, Upton RN, Milne RW. Inhibition of morphine metabolism by ketamine. Drug Metab Dispos. 2010; 38(5): 728-31[DOI][PubMed]
  • 14. Struck MB, Andrutis KA, Ramirez HE, Battles AH. Effect of a short-term fast on ketamine-xylazine anesthesia in rats. J Am Assoc Lab Anim Sci. 2011; 50(3): 344-8[PubMed]
  • 15. Reports funded by National Institutes of Health, Guide for the Care and Use of Laboratory Animals . Washington (DC); National Academies Press. 2011;
  • 16. Toedebusch RG, Childs TE, Hamilton SR, Crowley JR, Booth FW, Roberts MD. Postprandial leucine and insulin responses and toxicological effects of a novel whey protein hydrolysate-based supplement in rats. J Int Soc Sports Nutr. 2012; 9(1): 24[DOI][PubMed]
  • 17. Franco FS, Costa NM, Ferreira SA, Carneiro-Junior MA, Natali AJ. The effects of a high dosage of creatine and caffeine supplementation on the lean body mass composition of rats submitted to vertical jumping training. J Int Soc Sports Nutr. 2011; 8: 3[DOI][PubMed]
  • 18. Rusu D, Drouin R, Pouliot Y, Gauthier S, Poubelle PE. A bovine whey protein extract can enhance innate immunity by priming normal human blood neutrophils. J Nutr. 2009; 139(2): 386-93[DOI][PubMed]
  • 19. Rusu D, Drouin R, Pouliot Y, Gauthier S, Poubelle PE. A bovine whey protein extract stimulates human neutrophils to generate bioactive IL-1Ra through a NF-kappaB- and MAPK-dependent mechanism. J Nutr. 2010; 140(2): 382-91[DOI][PubMed]
  • 20. Maughan RJ. The Encyclopaedia of Sports Medicine: An IOC Medical Commission Publication, Nutrition in Sport. 2008;
  • 21. Jang TR, Wu CL, Chang CM, Hung W, Fang SH, Chang CK. Effects of carbohydrate, branched-chain amino acids, and arginine in recovery period on the subsequent performance in wrestlers. J Int Soc Sports Nutr. 2011; 8: 21[DOI][PubMed]
  • 22. Heinzen EL, Pollack GM. Pharmacokinetics and pharmacodynamics of L-arginine in rats: a model of stimulated neuronal nitric oxide synthesis. Brain Res. 2003; 989(1): 67-75[PubMed]
  • 23. Jahangir E, Vita JA, Handy D, Holbrook M, Palmisano J, Beal R, et al. The effect of L-arginine and creatine on vascular function and homocysteine metabolism. Vasc Med. 2009; 14(3): 239-48[DOI][PubMed]
  • 24. Miller RD, Eriksson LI, Fleisher LA, Wiener-Kronish JP, Young WL. Miller's Anesthesia. Philadelphia: Churchill Livingstone 2010;
  • 25. Barash PG. Clinical anesthesia. 2009;
  • 26. Albrecht M, Henke J, Tacke S, Markert M, Guth B. Effects of isoflurane, ketamine-xylazine and a combination of medetomidine, midazolam and fentanyl on physiological variables continuously measured by telemetry in Wistar rats. BMC Vet Res. 2014; 10: 198[DOI][PubMed]
  • 27. Yoshizawa F. New therapeutic strategy for amino acid medicine: notable functions of branched chain amino acids as biological regulators. J Pharmacol Sci. 2012; 118(2): 149-55[PubMed]
  • 28. Bihuniak JD, Insogna KL. The effects of dietary protein and amino acids on skeletal metabolism. Mol Cell Endocrinol. 2015; 410: 78-86[DOI][PubMed]
  • 29. Aparicio VA, Nebot E, Porres JM, Ortega FB, Heredia JM, Lopez-Jurado M, et al. Effects of high-whey-protein intake and resistance training on renal, bone and metabolic parameters in rats. Br J Nutr. 2011; 105(6): 836-45[DOI][PubMed]
  • 30. Vandenberg RJ, Ryan RM, Carland JE, Imlach WL, Christie MJ. Glycine transport inhibitors for the treatment of pain. Trends Pharmacol Sci. 2014; 35(8): 423-30[DOI][PubMed]
  • 31. Takagi N. Protein tyrosine phosphorylation in the ischemic brain. J Pharmacol Sci. 2014; 125(4): 333-9[PubMed]
  • 32. Koksal GM, Erbabacan E, Tunali Y, Karaoren G, Vehid S, Oz H. The effects of intravenous, enteral and combined administration of glutamine on malnutrition in sepsis: a randomized clinical trial. Asia Pac J Clin Nutr. 2014; 23(1): 34-40[DOI][PubMed]
  • 33. Kraemer WJ, Solomon-Hill G, Volk BM, Kupchak BR, Looney DP, Dunn-Lewis C, et al. The effects of soy and whey protein supplementation on acute hormonal reponses to resistance exercise in men. J Am Coll Nutr. 2013; 32(1): 66-74[DOI][PubMed]
  • 34. Dickinson H, Bain E, Wilkinson D, Middleton P, Crowther CA, Walker DW. Creatine for women in pregnancy for neuroprotection of the fetus. Cochrane Database Syst Rev. 2014; 12[DOI][PubMed]
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